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Molecular evolution of the SARS coronavirus during the course of the SARS epidemic in China.

Identifieur interne : 005236 ( Main/Exploration ); précédent : 005235; suivant : 005237

Molecular evolution of the SARS coronavirus during the course of the SARS epidemic in China.

Auteurs :

Source :

RBID : pubmed:14752165

Descripteurs français

English descriptors

Abstract

Sixty-one SARS coronavirus genomic sequences derived from the early, middle, and late phases of the severe acute respiratory syndrome (SARS) epidemic were analyzed together with two viral sequences from palm civets. Genotypes characteristic of each phase were discovered, and the earliest genotypes were similar to the animal SARS-like coronaviruses. Major deletions were observed in the Orf8 region of the genome, both at the start and the end of the epidemic. The neutral mutation rate of the viral genome was constant but the amino acid substitution rate of the coding sequences slowed during the course of the epidemic. The spike protein showed the strongest initial responses to positive selection pressures, followed by subsequent purifying selection and eventual stabilization.

DOI: 10.1126/science.1092002
PubMed: 14752165


Affiliations:


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Le document en format XML

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<term>Carnivora (virology)</term>
<term>China (epidemiology)</term>
<term>Cluster Analysis</term>
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<term>Adaptation, Physiological</term>
<term>Amino Acid Substitution</term>
<term>Animals</term>
<term>Base Sequence</term>
<term>Cluster Analysis</term>
<term>Disease Outbreaks</term>
<term>Evolution, Molecular</term>
<term>Genome, Viral</term>
<term>Genotype</term>
<term>Humans</term>
<term>Molecular Sequence Data</term>
<term>Mutation</term>
<term>Nucleic Acid Conformation</term>
<term>Open Reading Frames</term>
<term>Phylogeny</term>
<term>Point Mutation</term>
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<term>Analyse de regroupements</term>
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<term>Conformation d'acide nucléique</term>
<term>Données de séquences moléculaires</term>
<term>Délétion de séquence</term>
<term>Flambées de maladies</term>
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<div type="abstract" xml:lang="en">Sixty-one SARS coronavirus genomic sequences derived from the early, middle, and late phases of the severe acute respiratory syndrome (SARS) epidemic were analyzed together with two viral sequences from palm civets. Genotypes characteristic of each phase were discovered, and the earliest genotypes were similar to the animal SARS-like coronaviruses. Major deletions were observed in the Orf8 region of the genome, both at the start and the end of the epidemic. The neutral mutation rate of the viral genome was constant but the amino acid substitution rate of the coding sequences slowed during the course of the epidemic. The spike protein showed the strongest initial responses to positive selection pressures, followed by subsequent purifying selection and eventual stabilization.</div>
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